969084.fig.001a
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969084.fig.001b
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969084.fig.001c
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Figure 1: Breast carcinoma effusion and primary carcinoma expression arrays. (a): Volcano plot of differentially expressed genes in malignant effusions in comparison to pooled primary tumors (n=6). The x-axis indicates the differential expression profiles, plotting the fold-induction ratios in a log-2 scale. The y-axis indicates the statistical significance of the difference in expression (P-value, t-test) in a -log10 scale. Differentially expressed genes (P<.05) appear above the horizontal line. Numbers denote up-regulated (red) or down-regulated (blue) genes in effusions. (b): Gene expression profiling of effusions (blue) and primary breast carcinomas (red) in three-dimensional space by Principal Component Analysis using 351 genes that showed significant up- or downregulation in effusions in comparison to the pooled primary tumor sample (two different view angles). (c): Gene expression profiling of 11 effusions (blue) and 11 primary carcinomas (red) in three-dimensional space by Principal Component Analysis (three different view angles).