561862.fig.002a
(a)
561862.fig.002b
(b)
561862.fig.002c
(c)
Figure 2: Cell cycle analysis of proliferating A549 cells. Histogram plot (Event count versus FL2-H) of AECS/p-BQ-induced proliferation of A549 cells and its prevention by vitamin C as determined by propidium iodide (PI) staining followed by flow cytometry (a). Bar diagram showing mean fluorescence of PI (FL2-H) in cells either nontreated (NT) or exposed to 2 μL/mL AECS or 200 ng/mL p-BQ for 24 hr and its prevention by vitamin C (40 μg/mL) (b). Bar diagram showing the relative cell distribution during various phases of the cell cycle after exposure to 2 μL/mL AECS or 200 ng/mL p-BQ (c). Data are means ± SD for three independent experiments (* in (b) and (c) indicates significant difference, in comparison to AECS and p-BQ treatment).