Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma
Figure 2
CSC Identification. (a) Tumor sphere formation by CSCs. Differentiated tumor cells (pink) are unable to give rise to new clonally derived tumor spheres, whereas CSCs (green) give rise to new tumor spheres, (b) Tumor subpopulations are identified through differing mechanisms, including cell surface markers, aldehyde dehydrogenase activity, side population and are isolated with fluorescence-activated cell sorting (FACS). Xenotransplantation assays in immunocompromised mice demonstrate tumorigenic, self-renewing and differentiation properties of putative CSC population.
