Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities
Figure 2
(a) Summary of Multiple Cloning Experiments by normal & CML. Total CD34+ Cells and G0 and G1/S/G2/M fractions. Comparison of average results of multiple cloning experiments (total ) comparing total cloning efficiencies of CML and normal total CD34+ cells and G0 and G1/S/G2/M subsets with different cytokine combinations: KL + FL = TPO Experiments; KL + G-CSF + GM-CSF ; and KL + FL + TPO + G-CSF + GM-CSF ± IL-3 ± IL-6 . (b) Cloning of CD34+ G0 Cells from 2 normal bone marrow samples. Cloning of 2 normal CD34+ G0 cells enriched from fresh bone marrow samples from 2 normal volunteers using the color scale of the estimated colony sizes of numbers of cells per colony according to the standard scale described under methods. The CFU-GM and erythroid (CFU-E/BFU-E) clusters and colonies of all sizes are designated, respectively, CFU-GM and BFU-E on this and subsequent graphs. C.E. = %total Cloning Efficiency. (c) Cloning of 2 CML CD34+ G0 cells enriched from frozen-thawed mononuclear cells obtained from the peripheral blood of 2 CML patients in chronic phase.
