Gene Expression Differences between Enriched Normal and Chronic Myelogenous Leukemia Quiescent Stem/Progenitor Cells and Correlations with Biological Abnormalities

Table 1

Numbers of G0 and G1/S/G2/M cells recovered from highly enriched normal and CML CD34+ population. In the upper part of the table are shown the data showing the recovery of cells from normal bone marrows and in the lower part the recovery of cells from the CML patients. In bold is the average recovery of each fraction relative to the CD34+ starting population. The last column shows the percentage of blasts from each patient at the moment of sample collection, and the asteriks indicate the patients in accelerated phase (more than 5% of blasts). Cells recovered from BM #2 were not used due to the insufficient number of cells recovered. Legend: starting CD34+ cells in millions (S-CD34+), CD34+ G1/S/G2/M cells recovered (R-CD34+ G1/S/G2/M), CD34+ G1/S/G2/M cells recovered as % of starting CD34+ cells (%G1/S/G2/M), CD34+ G0 cells recovered (R-G0), and CD34+ G0 cells recovered as % of starting CD34+ cells (% G0).

Normal BM	S-CD34+	R-G1/S/G2/M	%G1/S/G2/M	R-G0	%G0	% blasts
1	2.55	400,000	15.3	50,000	1.96	—
2	0.5	64,000	12.8	19,400	3.9	—
3	5.85	1,000,000	17.1	400,000	6.84	—
4	2.8	370,000	13.2	73,000	2.56	—
5	3.6	1,170.00	32	238,000	6.6	—
		𝐌𝐞𝐚𝐧=𝟑.𝟎𝟔%	𝐌𝐞𝐚𝐧=𝟏𝟖.𝟎%		𝐌𝐞𝐚𝐧=𝟒.𝟑%
CML patient	S-CD34+	R-G1/S/G2/M	%G1/S/G2/M	R-G0	%G0	% Blasts
1	6	580,000	9.6	100,000	1.6	2%
2	3.5	387,000	11	123,000	3.51	1%
3	0.9	230,000	25.5	42,000	4.6	<3%
4	1.7	320,000	18.8	74,800	4.4	3%
5	4.25	310,000	7.29	150,000	3.52	1%
6	4.59	300,000	6.53	150,000	3.26	*18%
7	0.85	158,000	18.5	13,500	1.58	*14%
8	3.45	719,000	20.8	68,700	1.99	*8.5%
	𝐌𝐞𝐚𝐧=𝟑.𝟏𝟓%		𝐌𝐞𝐚𝐧=𝟏𝟒.𝟕%		𝐌𝐞𝐚𝐧=𝟑.𝟐%