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Journal of Oncology
Volume 2011, Article ID 819129, 13 pages
http://dx.doi.org/10.1155/2011/819129
Review Article

Cigarette Smoke, Bacteria, Mold, Microbial Toxins, and Chronic Lung Inflammation

Department of Immunology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA

Received 16 November 2010; Revised 28 February 2011; Accepted 20 March 2011

Academic Editor: Venkateshwar Keshamouni

Copyright © 2011 John L. Pauly and Geraldine Paszkiewicz. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. M. Borgerding and H. Klus, “Analysis of complex mixtures—cigarette smoke,” Experimental Toxicology and Pathology, vol. 57, supplement 1, pp. 43–73, 2005. View at Google Scholar
  2. R. R. Baker, “Smoke chemistry,” in TOBACCO: Production, Chemistry and Technology, D. Layten Davis and M. T. Nielsen, Eds., charter 12, pp. 398–439, Blackwell Science, 2003. View at Google Scholar
  3. S. S. Hecht, “Cigarette smoking: cancer risks, carcinogens, and mechanisms,” Langenbeck's Archives of Surgery, vol. 391, no. 6, pp. 603–613, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  4. A. Rodgman and T. A. Perfetti, The Chemical Components of Tobacco and Tobacco Smoke, CCRC Press, Taylor and Francis Group, Boca Raton, Fla, USA, 2009.
  5. D. Hoffmann and I. Hoffmann, “The changing cigarette, 1950–1995,” Journal of Toxicology and Environmental Health A, vol. 50, no. 4, pp. 307–364, 1997. View at Google Scholar · View at Scopus
  6. G. F. Wayne and G. N. Connolly, “Regulatory assessment of brand changes in the commercial tobacco product market,” Tobacco Control, vol. 18, no. 4, pp. 302–309, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  7. D. M. Burns and C. M. Anderson, “Do changes in cigarette design influence the rise in adenocarcinoma of the lung?” Cancer Causes Control, vol. 22, pp. 13–22, 2011. View at Google Scholar
  8. R. J. O'Connor, K. M. Cummings, V. W. Rees et al., “Surveillance methods for identifying, characterizing, and monitoring tobacco products: potential reduced exposure products as an example,” Cancer Epidemiology Biomarkers and Prevention, vol. 18, no. 12, pp. 3334–3348, 2009. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  9. D. K. Hatsukami, K. A. Perkins, M. G. LeSage et al., “Nicotine reduction revisited: science and future directions,” Tobacco Control, vol. 19, no. e1, pp. 1–10, 2010. View at Google Scholar
  10. H. Ito, K. Matsuo, H. Tanaka et al., “Nonfilter and filter cigarette consumption and the incidence of lung cancer by histological type in Japan and the United States: analysis of 30-year data from population-based cancer registries,” International Journal of Cancer, vol. 128, no. 8, pp. 1918–1928, 2011. View at Google Scholar
  11. M. Rabinoff, N. Caskey, A. Rissling, and C. Park, “Pharmacological and chemical effects of cigarette additives,” American Journal of Public Health, vol. 97, no. 11, pp. 1981–1991, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  12. Brown & Williamson, “Commonly added ingredients,” 1982, Bates Number 521057548/7553. Retrieved on February 22. 2011 from http://legacy.library.ucsf.edu/tid/tmx33f00.
  13. A Report of the Surgeon General, “How tobacco smoke causes disease. The biology and behavior basis for smoking-attributable disease,” U.S. Department of Health and Human Services, Atlanta, GA; Office on Smoking and Health, U.S. Government Printing Office, Washington, DC 20402, 704 pgs, 2010. http://www.cdc.gov/tobacco/data_statistics/sgr/2010/index.htm.
  14. A. M. Bromnawell, Reports of the Life Science Research Office (LSRO), Bethesda, MD, Volume I. Biological effects assessment in the evaluation of potential reduced-risk tobacco products, 242 pages; Volume II. Scientific methods to evaluate potential reduced-risk tobacco products, 174 pages; and Volume III. Exposure assessment in the evaluation of potential reduced-risk tobacco products, 170 pages, 2007.
  15. L. P. Carter, M. L. Stitzer, J. E. Henningfield, R. J. O'Connor, K. M. Cummings, and D. K. Hatsukam, “Review: abuse liability assessment of tobacco products including potential reduced exposure products,” Cancer Epidemiology Biomarkers and Prevention, vol. 18, no. 12, pp. 3241–3262, 2009. View at Google Scholar
  16. J. Beherns, “Die beziehungen der microorganisms zum tabaksbau and zur tabakferntation,” Zentrabl Bakteriol Parasitenk, Abt II, vol. 2, pp. 514–527, 1896. View at Google Scholar
  17. Anonymous, “Tobacco and bacteria,” The London Globe, pp. 7, July 21, 1899. Retrieved on November 11, 2010 from http://query.nytimes.com/gst/abstract.html?res=FA0F14F63F5414728DDDA80A94DF405B8985F0D3.
  18. H. Okino, W. C. Squires, and R. J. Reynolds, “Microbial degradation of nicotine and nicotinic acid. Part I, Isolation of nicotine decomposing bacteria and their morphological and physiological properties,” 1954. Bates number 508893294/3298. Retrieved on June 24, 2011 from http://legacy.library.ucsf.edu/tid/xwr83d00.
  19. A. Wiernik, A. Christakopoulos, L. Johansson, and I. Wahlberg, “Effect of air-curing on the chemical composition of tobacco,” Recent Advances in Tobacco Science, vol. 21, pp. 39–80, 1955. View at Google Scholar
  20. H. P. Dygert, “Snuff-a source of pathogenic bacteria in chronic bronchitis,” The New England Journal of Medicine, vol. 257, no. 7, pp. 311–313, 1957. View at Google Scholar
  21. W. K. Farr and A. Revere, Examination of Whole Cigarette Smoke by Light and Electron Microscopy, Life Extension Foundation, New York, NY, USA, 1958.
  22. W. K. Farr and A. Revere, “Examination of whole cigarette smoke by light and electron microscopy,” Journal of the American Medical Association, vol. 172, no. 4, p. 405, 1960. View at Google Scholar
  23. J. Forgacs and W. T. Carll, “Mycotoxicoses: toxic fungi in tobaccos,” Science, vol. 152, no. 3729, pp. 1634–1635, 1966. View at Google Scholar
  24. W. A. Curby, “A preliminary study of the biological activity in cigarette smoke,” 1967, Bates Number 11330877-0905. Retrieved on March 23, 2011 from http://legacy.library.ucsf.edu/tid/jtp6aa00.
  25. W. A. Curby, “A preliminary study of the biological activity in cigarette smoke—part II,” 1967, Bates Number 11330942-0973. Retrieved on March 23, 2011 from http://legacy.library.ucsf.edu/tid/stp6aa00.
  26. R. E. Welty, “Fungi isolated from flue-cured tobacco sold in Southeast United States, 1968–1970,” Applied Microbiology, vol. 24, no. 3, pp. 518–520, 1972. View at Google Scholar
  27. T. G. Mitchell and British-American Tobacco Company, “Microbiological examination of tobacco products: report Number RD 969-R,” 1972, Bates number 105501740/1767. Retrieved on June 28, 2010 from http://legacy.library.ucsf.edu/tid/xwp67a99.
  28. P. C. Stauber and British American Tobacco Company, “Microbiology of Henri Wintermans cigar production on-site studies of the primary process at Eersel: report No. RD 925R,” 1972, Bates number 107466852/6877. Retrieved on June 28, 2010 from http://legacy.library.ucsf.edu/tid/dpe66a99.
  29. T. G. Mitchell and P. C. Stauber, “Methods for the microbiological examination of tobacco and tobacco products, Report Number 888—R,” 1972, Bates number 105597063/7412. Retrieved on July 22, 2010 from http://legacy.library.ucsf.edu/tid/vit379.
  30. Smoke Study Group, “CORESTA—Papers presented at the Kallithea Symposium,” 1991, Bates number 2021551986/2194. Retrieved on July 27, 2010 from http://legacy.library.ucsf.edu/tid/zhe58e00.
  31. I. Rubinstein and G. W. Pederson, “Bacillus species are present in chewing tobacco sold in the United States and evoke plasma exudation from the oral mucosa,” Clinical Diagnostics Laboratory and Immunology, vol. 9, pp. 1057–1060, 1992. View at Google Scholar
  32. R. L. Barnes and S. A. Glantz, “Endotoxins in tobacco smoke: shifting tobacco industry positions,” Nicotine and Tobacco Research, vol. 9, no. 10, pp. 995–1004, 2007. View at Publisher · View at Google Scholar · View at PubMed
  33. A. Morin, F. Samson, A. Porter, and J. Torrie, “Development of an easy to-search database on the microbes associated with tobacco,” 1990 Bates number 620693477/3480. Retrieved on Nov. 8, 2010 from http://tobaccodocuments.org/rjr/522305212-5212.html.
  34. J. D. Hasday, R. Bascom, J. J. Costa, T. Fitzgerald, and W. Dubin, “Bacterial endotoxin is an active component of cigarette smoke,” Chest, vol. 115, no. 3, pp. 829–835, 1999. View at Publisher · View at Google Scholar
  35. K. S. Lane, “Method and system for assay and removal of harmful toxins during processing of tobacco products,” US patent 6,786,221. September 7, 2004.
  36. L. Larsson, B. Szponar, and C. Pehrson, “Tobacco smoking increases dramatically air concentrations of endotoxin,” Indoor Air, vol. 14, no. 6, pp. 421–424, 2004. View at Publisher · View at Google Scholar · View at PubMed
  37. W. P. Hempling, G. H. Bokelman, and M. Shulleeta, “Method for reduction of tobacco specific nitrosamines,” US patent 6,755,200, June 29, 2004.
  38. A. P. Rooney, J. L. Swezey, D. T. Wicklow, and M. J. McAtee, “Bacterial species diversity in cigarettes linked to an investigation of severe pneumonitis in U.S. military personnel deployed in Operation Iraqi Freedom,” Current Microbiology, vol. 51, no. 1, pp. 46–52, 2005. View at Publisher · View at Google Scholar · View at PubMed
  39. A. Sebastian, C. Pehrson, and L. Larsson, “Elevated concentrations of endotoxin in indoor air due to cigarette smoking,” Journal of Environmental Monitoring, vol. 8, no. 5, pp. 519–522, 2006. View at Publisher · View at Google Scholar · View at PubMed
  40. M. Zhao, B. Wang, F. Li et al., “Analysis of bacterial communities on aging flue-cured tobacco leaves by 16S rDNA PCR-DGGE technology,” Applied Microbiology and Biotechnology, vol. 73, no. 6, pp. 1435–1440, 2007. View at Publisher · View at Google Scholar · View at PubMed
  41. L. Larsson, B. Szponar, B. Ridha et al., “Identification of bacterial and fungal components in tobacco and tobacco smoke,” Tobacco Induced Diseases, vol. 4, no. 4, 2008. View at Google Scholar
  42. J. L. Pauly, J. D. Waight, and G. M. Paszkiewicz, “Tobacco flakes on cigarette filters grow bacteria: a potential health risk to the smoker?” Tobacco Control, vol. 17, supplement 1, pp. i49–i52, 2008. View at Google Scholar
  43. J. Yang, J. Yang, Y. Duan et al., “Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S rRNA sequence analysis,” Applied Microbiology and Biotechnology, vol. 88, pp. 553–562, 2010. View at Google Scholar
  44. L. M. Coussens and Z. Werb, “Inflammation and cancer,” Nature, vol. 420, no. 6917, pp. 860–867, 2002. View at Publisher · View at Google Scholar · View at PubMed
  45. O. Takeuchi and S. Akira, “Pattern recognition receptors and inflammation,” Cell, vol. 140, no. 6, pp. 805–820, 2010. View at Publisher · View at Google Scholar · View at PubMed
  46. M. Karin, T. Lawrence, and V. Nizet, “Innate immunity gone awry: linking microbial infections to chronic inflammation and cancer,” Cell, vol. 124, no. 4, pp. 823–835, 2006. View at Publisher · View at Google Scholar · View at PubMed
  47. C. Nathan and A. Ding, “Nonresolving inflammation,” Cell, vol. 140, no. 6, pp. 871–882, 2010. View at Publisher · View at Google Scholar · View at PubMed
  48. N. Azad, Y. Rojanasakul, and V. Vallyathan, “Inflammation and lung cancer: roles of reactive oxygen/nitrogen species,” Journal of Toxicology and Environmental Health B, vol. 11, no. 1, pp. 1–15, 2008. View at Publisher · View at Google Scholar · View at PubMed
  49. L. Zitvogel, O. Kepp, and G. Kroemer, “Decoding cell death signals in inflammation and immunity,” Cell, vol. 140, no. 6, pp. 798–804, 2010. View at Publisher · View at Google Scholar · View at PubMed
  50. K. E. de Visser and L. M. Coussens, “The inflammatory tumor microenvironment and its impact on cancer development,” Contributions to Microbiology, vol. 13, pp. 118–137, 2006. View at Google Scholar
  51. J. K. Kundu and Y. J. Surh, “Inflammation: gearing the journey to cancer,” Mutation Research, vol. 659, no. 1-2, pp. 15–30, 2008. View at Publisher · View at Google Scholar · View at PubMed
  52. E. A. Engles, “Inflammation in the development of lung cancer: epidemiological evidence,” Expert Review of Anticancer Therapy, vol. 8, no. 4, pp. 605–615, 2008. View at Google Scholar
  53. A. I. D'hulst, K. Y. Vermaelen, G. G. Brusselle, G. F. Joos, and R. A. Pauwels, “Time course of cigarette smoke-induced pulmonary inflammation in mice,” European Respiratory Journal, vol. 26, no. 2, pp. 204–213, 2005. View at Publisher · View at Google Scholar · View at PubMed
  54. C. Smith, T. Perfetti, and J. King, “Perspectives on pulmonary inflammation and lung cancer risk in cigarette smokers,” Inhalation Toxicology, vol. 18, no. 9, pp. 667–677, 2006. View at Publisher · View at Google Scholar · View at PubMed
  55. H. Van Der Vaart, D. S. Postma, W. Timens, and N. H. T. Ten Hacken, “Acute effects of cigarette smoke on inflammation and oxidative stress: a review,” Thorax, vol. 59, no. 8, pp. 713–721, 2004. View at Publisher · View at Google Scholar · View at PubMed
  56. M. A. Birrell, S. Wong, M. C. Catley, and M. G. Belvisi, “Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages,” Journal of Cellular Physiology, vol. 214, no. 1, pp. 27–37, 2008. View at Publisher · View at Google Scholar · View at PubMed
  57. L. Sorokin, “The impact of the extracellular matrix on inflammation,” Nature Reviews Immunology, vol. 10, no. 10, pp. 712–723, 2010. View at Google Scholar
  58. W. Huvenne, C. A. Pérez-Novo, L. Derycke et al., “Different regulation of cigarette smoke induced inflammation in upper versus lower airways,” Respiratory Research, vol. 11, no. 110, pp. 1–9, 2010. View at Google Scholar
  59. G. S. Kulkarni, P. P. Nadkarni, J. M. Cerreta, S. Ma, and J. O. Cantor, “Short-term cigarette smoke exposure potentiates endotoxin-induced pulmonary inflammation,” Experimental Lung Research, vol. 33, no. 1, pp. 1–13, 2007. View at Publisher · View at Google Scholar · View at PubMed
  60. E. Doz, N. Noulin, E. Boichot et al., “Cigarette smoke-induced pulmonary inflammation is TLR4/MyD88 and IL-1R1/MyD88 signaling dependent,” Journal of Immunology, vol. 180, no. 2, pp. 1169–1178, 2008. View at Google Scholar
  61. M. R. Stämpfli and G. P. Anderson, “How cigarette smoke skews immune responses to promote infection, lung disease and cancer,” Nature Reviews Immunology, vol. 9, no. 5, pp. 377–384, 2009. View at Publisher · View at Google Scholar · View at PubMed
  62. H. Mehta, K. Nazzal, and R. T. Sadikot, “Cigarette smoking and innate immunity,” Inflammation Research, vol. 57, no. 11, pp. 497–503, 2008. View at Publisher · View at Google Scholar · View at PubMed
  63. M. Sopori, “Effects of cigarette smoke on the immune system,” Nature Reviews Immunology, vol. 2, no. 5, pp. 372–377, 2002. View at Google Scholar
  64. J. Domagala-Kulawik, “Effects of cigarette smoke on the lung and systemic immunity,” Journal of Physiology and Pharmacology, vol. 59, no. 6, pp. 19–34, 2008. View at Google Scholar
  65. D. G. Yanbaeva, M. A. Dentener, E. C. Creutzberg, G. Wesseling, and E. F. M. Wouters, “Systemic effects of smoking,” Chest, vol. 131, no. 5, pp. 1557–1566, 2007. View at Publisher · View at Google Scholar · View at PubMed
  66. D. Wood, “British-American Tobacco Company,” Preliminary observations on the possible transfer of viable micro-organisms to mainstream smoke, 1968, Bates number 570343882/3901. Retrieved on June 24, 2011 from http://legacy.library.ucsf.edu/tid/jnd51f00.
  67. J. Forgacs, Good Samaritan Hospital, Suffern, NY to Dr. Robert C. Hockett, Council for Tobacco Research, New York, NY, 2 pages, 2010, http://legacy.library.ucsf.edu/tid/ppd2aa00.
  68. J. Forgacs, “Mycotoxicoses: the neglected diseases,” Feedstuffs, vol. 36, no. 18, pp. 124–134, 1966. View at Google Scholar
  69. B. Slutzker, G. Harmon, and P. Edmonds, “Microbiological content of tobacco smoke,” The American Journal of the Medical Sciences, vol. 243, pp. 196–201, 1962. View at Google Scholar
  70. J. M. Greene and S. Caldwell, “Chemical and microbiological changes during flue curing of NK-149 tobacco,” 1989, R. J. Reynolds, Bates number 514848867/8887, retrieved on June 30, 2010 from http://legacy.library.ucsf.edu/tid/qlm03d00.
  71. Anonymous, Brown & Williamson, “Package 13.0 microbiology,” No date. Bates number 620648956/9146. Retrieved on June 28, 2010 from http://legacy.library.ucsf.edu/tid/key21f00.
  72. Anonymous, British American Tobacco, “Master RD 888-R,” Bates number 105597011/7062. Retrieved on December 11, 2009 from http://legacy.library.ucsf.edu/tid/unit 37a99.
  73. C. W. Bacon, R. Wenger, and J. F. Bullock, “Chemical changes in tobacco during flu-curing,” Industrial & Engineering Chemistry, vol. 44, no. 2, pp. 292–296, 1952. View at Google Scholar
  74. C. O. Jensen, “Uber die natur der tabakfermentation,” Zentrabl Bakteriol Parasitenk, Abt II, vol. 21, pp. 469–483, 1908. View at Google Scholar
  75. W. P. Hempling and P. Morris, “Fundamental tobacco microbiology,” 1987. Bates number 2022226783/6795. Retrieved on March 24, 2010 from http://legacy.library.ucsf.edu/tid/jst58e00.
  76. K. J. Brotzge and Brown and Williamson Tobacco Company, Quantities of microflora recovered from Brown & Williamson & competitive cigarette brands, Fall/Winter, 1982, 1983. Bates number 598000442/0451. Retrieved on March 16, 2010 from http://tobaccodocuments.org/bw/971381.html.
  77. K. J. Brotzge and Brown & Williamson Tobacco Company, “Quantities of microflora recovered from Brown & Williamson and competitive brands, Spring/Summer 830000,” 1983, Bates number 657017733/7752. Retrieved on March 16, 2010 from http://legacy.library.ucsf.edu/tid/hnl13f00.
  78. L. J. Dewey and G. M. Broaddus, “Bacterial, mold and yeast population counts on RCFS and on RC picked from Pall Mall and Winston cigarettes,” 1970, American Tobacco Company, Bates number 950107079/7080, Retrieved on March 16, 2010 from http://legacy.library.ucsf.edu/tid/jml11a00.
  79. J. Hill and Brown & Williamson, “Microflora standards of cocoa casing materials,” 1985. Bates number 62018442/4422. Retrieved on March 17, 2010 from http://legacy.library.ucsf.edu/tid/ski31f00.
  80. M. I. Hofer and Philip Morris, “Research and development—quarterly Report Microbiology 851000/1200,” 1985, Bates number 2028639252/9269. Retrieved on November 8, 2010 from http://legacy.library.ucsf.edu/tid/rew56e00.
  81. Philip Morris, “Biocontrol of tobacco microflora,” 1989, Bates number 2029139024/9050, retrieved on June 30, 2010 from http://legacy.library.ucsf.edu/tid/hoy69e00.
  82. R. E. Welty and American Tobacco, “Plant pathology, 5225, microflora of flue-cured tobacco and their affect on quality,” 1970, Bates number 950251672/1675. Retrieved on June 30, 2010 from http://legacy.library.ucsf.edu/tid/ufv31A00.
  83. Anonymous and Philip Morris, “Most populous bacteria: burley tobacco research,” 1999, Bates number 2082730005. Retrieved on March 19, 2010 from http://legacy.library.ucsf.edu/tid/ddq55c00.
  84. Anonymous, “Further examination of coliform bacteria from cigarettes,” Laboratory report L.337-R. 1970, Bates number 650018029/8046. Retrieved on June 24, 2010 from http://legacy.library.ucsf.edu/tid/rkl66b00.
  85. M. Di Giacomo, M. Paolino, D. Silvestro et al., “Microbial community structure and dynamics of dark fire-cured tobacco fermentation,” Applied and Environmental Microbiology, vol. 73, no. 3, pp. 825–837, 2007. View at Publisher · View at Google Scholar · View at PubMed
  86. T. G. Mitchell and British American Tobacco (BAT), “Changes in the microflora of tobacco leaves during field growth in England,” 1989, Bates number 400047269/7282, retrieved on June 28, 2010 from http://legacy.library.ucsf.edu/tid/num81a99.
  87. S. A. Ghabrial, “Studies on the microflora of air-cured burly tobacco,” Tobacco Science, vol. 20, pp. 80–82, 1976. View at Google Scholar
  88. British American Tobacco, “Film Box Number -1, L1R to L151 R, R&D 1838,” Bates number 402185400/5586, Retrieved on June 24, 2010 from http://legacy.library.ucsf.edu/tid/fud91a99.
  89. W. C. Squires, L. E. Hayes, and R. J. Reynolds, “Tobacco flora: quantitative studies,” November 9, 1961. 125 pages/ Retrieved on October 27, 2010 from http://tobaccodocuments.org/rjr/500937365-7489.html.
  90. V. Subbiah, “Sheet2. Tobacco sampling for microflora counting,” 1995. Bates number 525450330/0335. Retrieved on October 22, 2010 from http://legacy.library.ucsf.edu/tid/ymt60d00.
  91. W. C. Flanders, R. J. Reynolds, “Quantitative studies of the microbiological flora of tobacco during aging,” 1955. Bates number 501663388/3456. Retrieved on July 1, 2010 from http://legacy.library.ucsf.edu/tid/wuk39d00.
  92. P. C. Stauber, “Investigation of mould growth on stored leaf,” 1975. Bates number 105425004/5072. Retrieved on June 24, 2011 from http://legacy.library.ucsf.edu/tid/oit57a99.
  93. R. E. Welty and L. A. Nelson, “Growth of aspergillus repens in flue-cured tobacco,” Applied Microbiology, vol. 21, no. 5, pp. 854–859, 1971. View at Google Scholar
  94. T. G. Mitchell, D. A. Johnson, and British-American Tobacco Company, “Identification of fungi of the Aspergillus flavour group from tobacco,” Report RD 1279. 1975. Bates number 105598328/8619. Retrieved on July 1, 2010 from http://library/ucsf/edu/tid/pnp57a99.
  95. G. M. Myers, “Aflatoxin on tobacco and its removal,” R. J. Reynolds, Bates number 519972600/2620. Retrieved on June 29, 2010 from http://legacy.library.ucsf.edu/tid/yjk90d00.
  96. J. M. Greene and S. Caldwell, “Chemical and microbiological changes during flue curing of NK-149 tobacco,” 1989, R. J. Reynolds, Bates number514848867/8887, Retrieved on June 30, 2010 from http://legacy.library.ucsf.edu/tid/qlm03d00.
  97. D. G. Vickroy and R. E. Welty, “Evaluations of cigarettes made with mold-damaged and nondamaged flue-cured tobacco,” Beiträge zur Tabakforschung, vol. 8, pp. 102–106, 1975. View at Google Scholar
  98. M. R. Tansey, “Isolation of thermophilic fungi from snuff,” Journal of Applied Microbiology, vol. 29, no. 1, pp. 128–129, 1975. View at Google Scholar
  99. T. Thomas, J. Brandon, W. A. Bailey, and T. A. Kosty, “Method for reducing nitrosamines in tobacco,” US patent 7,757,697. July 20, 2010.
  100. A. Lukic, R. E. Welty, and G. B. Lucas, “Antifungal spectra of actinomycetes isolated from tobacco,” Antimicrobial Agents and Chemotherapy, vol. 1, no. 4, pp. 363–366, 1972. View at Google Scholar
  101. K. Koga, S. Katsuya, and Japan Tobacco Company, “Method of reducing nitrosamines content in tobacco leaves,” US ptent 7,556,046. July 7, 2009.
  102. M. Cui, M. T. Nielsen, R. R. Hart III, M. L. Overbey, D. J. Watson, and J. R. Chipley, “Use of chlorate, sulfur or ozone to reduce tobacco specific nitrosamine,” U.S. patent 2006/019516 A1. Sept 7, 2006.
  103. R. G. Warke, A. S. Kamat, and M. Y. Kamat, “Irradiation of chewable tobacco mixes for improvement in microbiological quality,” Journal of Food Protection, vol. 62, no. 6, pp. 678–681, 1999. View at Google Scholar
  104. T. G. Mitchell and C. R. Jenkins, “Alternative treatments for mould control on pipe tobacco,” British American Tobacco, Bates number 400661432/1433. Retrieved on June 24, 2011 from http://legacy.library.ucsf.edu/tid/rir56a99.
  105. D. S. Roth, W. H. Cowart Jr., C. B. Jenkins Jr., and D. M. Boyle, “Sterilization process in the manufacturing of snuff,” U.S. patent 5,372,149. December 13, 1994.
  106. V. Subbiah, “Method of inhibiting mycotoxin production,” US Patent 5,698,599. Dec 16, 1997.
  107. R. P. Newton and Brown & Williamson, “Microbiological examination of cigarettes,” 1968. Retrieved on June 28, 2010 from http://legacy.library.ucsf.edu/tid/vgj94a99.
  108. V. C. Johnson, A. M. Palmer, and P. Morris, “Bacteria on cigarette filters,” Bates number 2000759148. February 12, 1968. Retrieved on November 9, 2010 from http://legacy.library.ucsf.edu/tid/aiw48e00.
  109. T. G. Mitchell and British-American Tobacco Company, “Examination of mould—Affected cigarettes from China,” 1989, Bates number 400910779/7783. Retrieved on June 24, 2010 from Legacy at http://legacy.library.ucsf.edu/tid/qvu0499.
  110. J. Hill and Brown & Williamson, “Microbial examination of pipe and smokeless tobacco/541,” 1985, Bates number 620184560/4571. Retrieved on July 22, 2010 from http://legacy.library.ucsf.edu/tid/rjq20f00.
  111. K. Brotzge and Brown & Williamson, “Microbial examination of pipe, snuff, & chewing tobacco products—Fall/Winter, 83000,” 1984, Bates number 598002147/2156. Retrieved on June 24, 2011 from http://legacy.library.ucsf.edu/tid/zcj41f00.
  112. S. K. Varma, A. B. Roy, and A. K. Jha, “Ecotoxicological aspects of Aspergilli present in the phylloplane of shored leaves of chewing tobacco (Nicotiana tobacum),” Mycopathologia, vol. 113, pp. 19–23, 1991. View at Google Scholar
  113. A. R. Sapkota, S. Berger, and T. M. Vogel, “Human pathogens abundant in the bacterial metagenome of cigarettes,” Environmental Health Perspectives, vol. 118, no. 3, pp. 351–356, 2010. View at Publisher · View at Google Scholar · View at PubMed
  114. J. Papavassiliou, G. Piperakis, and U. Marcelou-Kinti, “Mycological flora of cigarettes,” Mycopathology Mycology Applied, vol. 44, no. 2, pp. 117–120, 1971. View at Google Scholar
  115. V. P. Kurup, A. Resnick, S. L. Kagen, S. H. Cohen, and J. N. Fink, “Allergenic fungi and actinomycetes in smoking materials and their health implications,” Mycopathologica, vol. 82, no. 1, pp. 61–64, 1983. View at Google Scholar
  116. P. E. Verweij, J. J. Kerremans, A. Voss, and J. F. G. M. Meis, “Fungal contamination of tobacco and marijuana,” Journal of the American Medical Association, vol. 284, no. 22, p. 2875, 2000. View at Google Scholar
  117. N. B. Rainer, “Cigarettes having minimized loose ends and process for preparing same,” US patent 4,715,388, Dec 29, 1987.
  118. British American Tobacco Company, “Discussion group on ends quality,” 1985, 161 pages, http://tobaccodocuments.org/batco/109979765-9924.html.
  119. L. Deyton, J. Sharfstein, and M. Hamburg, “Tobacco product regulation—a public health approach,” The New England Journal of Medicine, vol. 362, no. 19, pp. 1753–1756, 2010. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus