Figure 3: Dietary restriction increases α-SMA and reduces Factor VIII expression in the CT-2A astrocytoma. The histograms show the average relative expression of the indicated protein normalized to β-actin based on Western blot analysis. Equal amounts of protein were loaded into each lane of the Western blot (25 μg). Other conditions were as described in Section 2. Values are expressed as normalized means of three to four independent tissue samples per group ± SEM. The value is significantly different in the tumors of DR-fed mice than in the tumors of AL-fed mice: * 𝑃 < 0 . 0 5 , Student’s t-test. Two representative samples are shown for each tissue type.