Figure 5: Dietary restriction reduces PDGF-Rβ and VEGF-R2 association in the CT-2A astrocytoma. (a) Confocal analysis of CT-2A tumor tissue double stained for VEGF-R2 (green) and PDGF-Rβ (red). Results show that the colocolization (yellow) of VEGF-R2 and PDGF-Rβ is less in the tumors of DR-fed mice than in the tumors of AL-fed mice. White arrow indicates colocolization. All other conditions are as described in Section 2. (b) The histogram shows that the average relative expression of PDGF-Rβ normalized to β-actin based on Western blot analysis is similar in the tumors of DR-fed and AL-fed mice. Equal amounts of protein were loaded into each lane (25 μg), and the other conditions were as described in Section 2. Values are expressed as normalized means of three to four independent tissue samples per group ± SEM. There is no significant difference between values of DR-fed and AL-fed mice, Student’s t-test. Two representative samples are shown for each tissue type.