Overexpression of NNMT in ccRCC tissues. (a) mRNA expression levels of NNMT of 33 tissue pairs of ccRCC measured by Q-PCR analysis. Expression levels are represented by values. Each dot represents one tissue sample, and horizontal bars show mean values. values are determined using a paired-samples Student’s test. (b) Western blot analysis to determine expression levels of NNMT protein in 10 ccRCC tissue pairs. N and T represent lysates from normal and tumor parts, respectively. Actin was used as a loading control.