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Journal of Oncology
Volume 2012, Article ID 796729, 14 pages
Research Article

Adamantyl Retinoid-Related Molecules Induce Apoptosis in Pancreatic Cancer Cells by Inhibiting IGF-1R and Wnt/β-Catenin Pathways

1John D Dingell VA MC, Wayne State University, Detroit, MI 48201, USA
2Department of Oncology, Wayne State University, Detroit, MI 48201, USA
3Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA
4Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA
5Department of Neurobiology, Harvard University, Cambridge, MA 02138, USA

Received 31 October 2011; Revised 17 January 2012; Accepted 30 January 2012

Academic Editor: Reidar Grenman

Copyright © 2012 Lulu Farhana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The CD44+/CD24+ cells were sorted by flow cytometry and approximately 200-300 cells were seeded with B27 medium in 96 well low attachment plates and 1 mM AHP3 added either the day after seeding or 7 days following spheroid formation. The sizes of spheroids were photographed and measured on a 100 mm scale and magnification 400 X using Olympus fluorescence microscope digital camera software and DP2-BSW software.

  1. Supplementary Figures