Towards a “Sample-In, Answer-Out” Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System

Table 1

NASBA chip results on clinical specimens. Six clinical specimens extracted on the sample preparation platform were tested on the NASBA chip platform for the dilutions 1 : 5 and 1 : 10. Positive amplification of target is denoted with “+”, while a negative result is denoted “−”. The table also shows the number of indeterminate reactions on chip, the number of channels filled and the overall amplification efficiency.

Sample			NASBA chip
Clinical specimen ID	HPV type	Dilution of eluate	+	−	Indeterminate	No. channels filled	Overall [% positive] channels filled
478	HPV16	1 : 5	1	6	0	7	14
478	HPV16	1 : 10	0	7	0	7	0
479	HPV33	1 : 5	0	0	8	8	0
479	HPV33	1 : 10	8	0	0	8	100
508	HPV16	1 : 5	1	7	0	8	12.5
508	HPV16	1 : 10	0	0	0	0	0
508	HPV33	1 : 5	7	0	0	7	100
508	HPV33	1 : 10	7	0	0	7	100
511	HPV16	1 : 5	4	4	0	8	50
511	HPV16	1 : 10	6	2	0	8	75
520	HPV16	1 : 5	4	0	0	4	100
520	HPV16	1 : 10	5	0	0	5	100
522	HPV16	1 : 5	3	1	1	5	60
522	HPV16	1 : 10	8	0	0	8	100
522	HPV33	1 : 5	0	6	0	6	0
522	HPV33	1 : 10	0	6	0	6	0