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Journal of Oncology
Volume 2013 (2013), Article ID 165014, 5 pages
Clinical Study

Circulating Prostate Cells Found in Men with Benign Prostate Disease Are P504S Negative: Clinical Implications

1Division of Medicine, Hospital Carabineros of Chile, Simón Bolívar 2200, Ñuñoa, 7770199 Santiago, Chile
2Instituto de BioOncología, Avenida Salvador 95, Oficina 95, Providencia, 7500710 Santiago, Chile
3Circulating Tumor Cell Unit, Faculty of Medicine Universidad Mayor, Renato Sánchez 4369, Las Condes, 7550224 Santiago, Chile
4Faculty of Medicine, Universidad Diego Portales, Manuel Rodriguez Sur 415, 8370179 Santiago, Chile
5Urology Service, Hospital Carabineros of Chile, Simón Bolívar 2200, Ñuñoa, 7770199 Santiago, Chile
6Radiotherapy, Fundación Arturo López Pérez, Rancagua 899, Providencia, 7500921 Santiago, Chile

Received 9 December 2012; Revised 3 March 2013; Accepted 23 March 2013

Academic Editor: Thomas Rutherford

Copyright © 2013 Nigel P. Murray et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. Developments in immunological and quantitative real-time PCR-based analysis have enabled the detection, enumeration, and characterization of circulating tumor cells (CTCs). It is assumed that the detection of CTCs is associated with cancer, based on the finding that CTCs can be detected in all major cancer and not in healthy subjects or those with benign disease. Methods and Patients. Consecutive men, with suspicion of prostate cancer, had blood samples taken before prostate biopsy; mononuclear cells were obtained using differential gel centrifugation and CPCs detecting using anti-PSA immunocytochemistry. Positive samples underwent further classification with anti-P504S. Results. 329 men underwent prostate biopsy; of these men 83 underwent a second biopsy and 44 a third one. Of those with a biopsy negative for cancer, 19/226 (8.4%) had CPCs PSA (+) P504S (−) detected at first biopsy, 6/74 (8.1%) at second biopsy, and 5/33 (15.2%) at third biopsy. Men with cancer-positive biopsies did not have PSA (+) P504S (−) CPCs detected. These benign cells were associated with chronic prostatitis. Conclusions. Patients with chronic prostatitis may have circulating prostate cells detected in blood, which do not express the enzyme P504S and should be thought of as benign in nature.