Research Article

Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin

Figure 3

The nuclear morphological changes induced by ENLE treatment at various time intervals on (a) MCF-7 (for 0, 48, and 72 h) and (b) HeLa (for 0, 24, and 48 h) cells. Untreated MCF-7 and HeLa cells (0 h) showed large and prominent nuclei, indicating no significant characteristics of apoptosis (white arrows). On the other hand, ENLE treatment of these cells at their respective EC50 induced time-dependent increase in nuclear morphological changes characteristic of apoptotic cells such as nuclear condensation and fragmentation (green arrows), nuclear marginalization (yellow arrows), and appearance of apoptotic bodies (pink arrows) (magnification 400x).