Research Article

AR-A 014418 Used against GSK3beta Downregulates Expression of hnRNPA1 and SF2/ASF Splicing Factors

Figure 5

Anxa7 splicing in U87 glioma. (a) Estimation of Anxa7 VA (variant) and Anxa7 VB (WT) in U373 and U87 glioma cells compared with Normal Brain cDNA. (b) Temporal kinetics of Anxa7 splicing (6, 12, and 24 hrs) in presence of AR-A 014418 (GSK3beta kinase inhibitor), Means S.E.; ; . (c) Small molecule against GSK3 kinase used to inhibit activated Tyrosine phosphorylated GSK3 kinase in U87 cells incubated for 24 hrs observed restoration of Annexin 7 tumor suppressor protein.
695325.fig.005a
(a)
695325.fig.005b
(b)
695325.fig.005c
(c)