Journal of Oncology / 2015 / Article / Fig 1

Research Article

Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA

Figure 1

Esculetin treatment downregulates the expression of AML1-ETO chimeric protein and C-Kit protein in Kausmi-1 cell line. Whole cell lysates from Kausmi-1 cells treated with 100 μM of esculetin for the indicated periods of time (in hours) were analysed. Equal amount of protein is loaded in each lane of the denaturing gel and immunoblotted with anti-AML1-ETO (a), anti-C-Kit (b), and anti-β-actin antibodies (c); β-actin was used as the loading control.