PPAR Enhances Cancer Cell Chemotherapy Sensitivity by Autophagy Induction

Figure 5

Agonist enhances autophagy in a PPAR-dependent manner. (a) Representative images of GFP-LC3 puncta (autophagosomes) in 10μM Clofibrate treated SW480 cells for 12 hours. Scar bar: 20 μm. The GFP-puncta was quantified. Results are expressed as means ± SEM (n=5). P<0.05. (b) SW480 cells were transfected with ctrl shRNA or PPARα shRNA. After 36 h, cells were treated with 10μM clofibrate for 12 hours. Cell lysates were subjected to Western blot. (c) SW480 cells were treated with 10μM clofibrate for 12 hours. Cell lysates were subjected to Western blot. Data are triplicates from three independent experiments.

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