Clinical Implication and the Hereditary Factors of NM23 in Hepatocellular Carcinoma Based on Bioinformatics Analysis and Genome–Wide Association Study

Figure 4

The association of NM23, STARD3 and PSORS1C1 in HCC patients. (a) Pathway network analysis for NM23, STARD3, and PSORS1C1 in GeneMANIA. (b) A scatter plot for the mRNA expression of NM23, STARD3, and PSORS1C1 between HCC and adjacent normal tissues. NM23 and STARD3 have a P<0.001, but PSORS1C1 has a P=0.056. (c) Correlation analysis for the mRNA expression between NM23 and PSORS1C1, r=-0.163, P=0.001. (d) Correlation analysis for the mRNA expression between NM23 and STARD3, r=0.259, and P=3.01 ×10⁻⁸. (e) Correlation analysis for the mRNA expression between PSORS1C1 and STARD3, r=-0.230, and P=9.42 ×10⁻⁷. All data are from the Gene Expression Omnibus (GEO accession: GSE14520). (f and g) LocusZoom plot for the analysis of local linkage disequilibrium (LD) and recombination patterns nearby PSORS1C1 (f) and STARD3 (g), ranging around 0.5 to 0.8 Mb. The vertical axis shows association P–values on the −log10 scale, and the right vertical axis shows the recombination rate. The horizontal axis shows the chromosomal position. The bottom of the plot shows the nearest gene. LD (r²) and recombination rates are estimated from the 1000 Genomes Project ASN data (March2012, build GRCh37/hg19). Haploview LD plot of PSORS1C1 SNPs (n=6) (f) and STARD3 SNPs (n=2) (g). Blocks represent SNPs in high LD. Greater color intensity and value inside each cell corresponds to a higher level of LD given by D’.