Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro
Table 1
Summary of differentially expressed proteins between SK-N-SH versus SK-N-AS secretomes (detailed information regarding peptide sequences and raw spot intensities in Supplementary Table 1).
Spot ID
Gene name
Protein name
SwissProt ID
Scores
Cov
No. of distinct peptides
pI
MW (kDa)
Spot intensity Mean±SEM
Fold change∗
p-value
SK-N-SH
SK-N-AS
Protein spots increased in SK-N-SH secretome
0
CCT6B
T-complex protein 1 subunit zeta-2
Q92526
81
2
1
6.85
58.35
0.245 ± 0.015
0.079 ± 0.011
3.10
1.59E-05
38
STMN1
Stathmin
P16949
154
9
2
5.76
17.29
0.580 ± 0.028
0.130 ± 0.022
4.43
1.43E-06
53
GSTP1
Glutathione S-transferase
P09211
46
5
1
5.43
23.57
0.418 ± 0.027
0.095 ± 0.011
4.41
3.60E-06
65
UCHL1
Ubiquitin carboxyl-terminal hydrolase isozyme L1
P09936
237
21
3
5.33
25.15
0.519 ± 0.062
0.145 ± 0.016
3.58
3.71E-04
67
TPI1
Triosephosphate isomerase
P60174
214
12
2
5.65
31.05
0.992 ± 0.124
0.202 ± 0.020
4.92
2.28E-04
72
YWHAE
14-3-3 protein epsilon
P62258
34
10
3
4.63
29.32
0.678 ± 0.024
0.168 ± 0.043
4.04
7.08E-06
75
PSMA8
Proteasome subunit alpha type-7-like
Q8TAA3
60
5
1
9.07
28.68
0.707 ± 0.081
0.119 ± 0.032
5.94
1.41E-04
85
HMGB1
High mobility group protein B1
P09429
217
13
2
5.62
25.04
0.354 ± 0.058
0.028 ± 0.010
12.83
5.74E-04
87
YWHAE
14-3-3 protein epsilon
P62258
158
11
3
4.63
29.32
0.279 ± 0.033
0.083 ± 0.017
3.37
7.60E-04
91
PSMA1
Proteasome subunit alpha type-1
P25786
227
17
3
6.15
29.82
0.119 ± 0.008
0.035 ± 0.004
3.43
1.67E-05
105
LDHA
L-lactate dehydrogenase A
P00338
115
11
3
8.44
36.95
0.572 ± 0.049
0.178 ± 0.028
3.22
1.17E-04
110
MDH2
Malate dehydrogenase, mitochondrial
P40926
375
17
4
8.92
35.90
0.894 ± 0.033
0.217 ± 0.031
4.11
4.07E-07
123
C11orf54
Ester hydrolase C11orf54
Q9H0W9
36
2
1
6.23
35.60
0.146 ± 0.014
0.044 ± 0.006
3.31
1.96E-04
134
GOT2
Aspartate aminotransferase, mitochondrial
P00505
149
6
2
9.14
47.88
0.282 ± 0.016
0.074 ± 0.019
3.82
3.36E-05
135
SET
SET protein
Q01105
94
7
2
4.23
33.47
0.407 ± 0.021
0.132 ± 0.017
3.07
7.65E-06
154
ENO2
Gamma-enolase
P09104
371
12
4
4.91
47.58
0.132 ± 0.014
0.041 ± 0.008
3.23
5.46E-04
158
ENO1
Alpha-enolase
P06733
243
11
3
7.01
47.48
1.390 ± 0.148
0.374 ± 0.039
3.72
1.62E-04
168
FKBP4
Peptidyl-prolyl cis-trans isomerase
Q02790
189
6
2
5.35
52.05
0.206 ± 0.009
0.051 ± 0.010
4.02
3.51E-06
183
HSP90AA1
Heat shock protein HSP 90-alpha
P07900
393
9
5
4.94
85.00
0.933 ± 0.125
0.101 ± 0.030
9.28
1.89E-04
184
VCP
Transitional endoplasmic reticulum ATPase
P55072
128
2
1
5.14
89.95
0.631 ± 0.114
0.131 ± 0.012
4.79
2.45E-03
223
GOT1
Aspartate aminotransferase, cytoplasmic
P17174
340
22
5
6.52
46.44
0.163 ± 0.009
0.041 ± 0.005
4.00
3.83E-06
241
HSPA4
Heat shock 70 kDa protein 4
P34932
185
3
2
5.11
95.12
0.114 ± 0.009
0.036 ± 0.007
3.14
1.70E-04
258
DBH
Dopamine beta-hydroxylase
P09172
191
5
2
5.97
69.87
0.230 ± 0.021
0.066 ± 0.012
3.48
1.37E-04
259
FH
Fumarate hydratase
P07954
296
8
2
8.85
54.77
0.123 ± 0.012
0.029 ± 0.056
4.15
9.83E-05
Protein spots decreased in SK-N-SH secretome
180
CHGA
Chromogranin-A
P10645
215
7
2
4.58
50.82
0.629 ± 0.091
2.092 ± 0.366
0.30
4.68E-03
205
B2M
Beta-2-microglobulin
P61769
261
37
2
6.06
13.82
0.105 ± 0.030
0.849 ± 0.082
0.12
2.71E-05
213
SPARC
SPARC
P09486
262
16
4
4.73
35.46
0.395 ± 0.070
1.816 ± 0.320
0.22
2.51E-03
235
ASS1
Argininosuccinate synthase
P00966
213
11
3
8.08
46.78
0.028 ± 0.003
0.163 ± 0.024
0.17
5.52E-04
237
IGFBP2
Insulin-like growth factor-binding protein 2
P18065
366
20
4
7.48
35.87
0.048 ± 0.005
0.184 ± 0.041
0.26
1.06E-02
242
C7
Complement component C7
P10643
455
9
4
6.09
96.65
0.411 ± 0.089
1.231 ± 0.190
0.33
4.53E-03
of the mean spot intensity of SK-N-SH group divided by SK-N-AS group. %Cov, the percentage of protein sequence covered by the identified peptides; MW, molecular weight; pI, isoelectric point.
