Journal of Oncology / 2019 / Article / Fig 3

Research Article

Plasticity of Drug-Naïve and Vemurafenib- or Trametinib-Resistant Melanoma Cells in Execution of Differentiation/Pigmentation Program

Figure 3

Changes of MITF-M level in melanoma cells treated with vemurafenib (PLX) or trametinib (TRA). (a) Drug influence on MITF-M transcript level. qRT-PCR data, normalized to the expression of a reference gene RPS17, are presented relative to control. (b) Drug-induced changes in MITF-M and SOX10 protein levels assessed by immunoblotting. GAPDH was used as loading control. The proteins were visualized by using ChemiDoc Imaging System (Biorad). The images are cropped, which is indicated by white spaces, whereas direct comparison of controls is shown in Figure 1(a). (c) Drug influence on the percentages of MITF-positive cells shown as representative density plots along with quantification from three independent experiments. Data are presented as mean ± SD. p < 0.05.

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