Dovitinib Triggers Apoptosis and Autophagic Cell Death by Targeting SHP-1/p-STAT3 Signaling in Human Breast Cancers

Figure 3

Blocking autophagy reduced the antitumor effects of dovitinib. (a) Cotreatment with the autophagy inhibitor, bafilomycin A1 (20 μmole/L), reduced the effect of dovitinib on cell death. Cells were treated with dovitinib at the indicated doses and/or bafilomycin A1 for 24 h. Cell viability was analyzed by MTT assay. Points, mean; bars, SD (N = 3). ; . (b) MCF-7 cells were treated with dovitinib at 15 μmol/L and/or bafilomycin A1 for 24 h. Cell viability was analyzed by MTT assay. Cell extracts were subjected to immunoblot analysis for LC3B and actin. Columns, mean; bars, SD (N = 3). ; .

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