Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor Angiogenesis

Figure 2

Low GAS5 expression in tissues, cells, and cell-derived exosomes of human lung cancer. (a) GAS5 expression in human lung cancer tissues and paracancerous tissues was detected by qPCR; GAPDH served as the internal reference. (b) GAS5 expression in human lung cancer cells was detected by qPCR; GAPDH served as the internal reference. (c) Representative images of isolated exosomes by electron microscopy. (d) Exosome marker CD63 was detected by western blot. (e) The GAS5 level of cell culture supernatant exosomes from lung cancer cell lines, cel-miR-39-3p served as the external reference. Statistical significance, P<0.05, P<0.01.

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