Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer
Figure 4
Detection and determination of FZD-10 expression levels in sEVs and in whole plasma samples of healthy donors and cancer patients by Western blotting and densitometry analysis. Representative Western blotting of FZD-10, two exosomal/EV protein markers (Hsp70 and ALIX) and GADH in sEVs, whole plasma and EVs-depleted plasma of healthy donors, CRC patient (before and after surgery), and GC patient (before surgery and after pathology resolution). Molecular mass markers indicated on the right. The same load (20 μg) of samples based on total protein content (a). Semiquantitative evaluation of relative FZD-10 expression in sEVs extracts and whole plasma by densitometry analysis of protein bands in (a). FZD-10 bands were measured, upon normalization with corresponding housekeeping Hsp70 and GAPDH bands in the case of sEVs and plasma samples, respectively, for each CRC, GC, and metastatic CRC patient (three replicates). Average value of FZD-10 expression levels among each group of subjects reported in graph. () p<0.005 versus healthy control. n= 16 for CRC, n=2for GC, n=6 for metastatic CRC, and n=8 for healthy donors (b).