Screening of Pleural Mesothelioma Cell Lines for Kinase Activity May Identify New Mechanisms of Therapy Resistance in Patients Receiving Platin-Based Chemotherapy

Figure 1

Heatmap of phosphorylation pattern between cells and treatment. MRC-5 is depicted by the green indicator on the y-axis and shows minor phosphorylation changes when comparing cisplatin treatment and medium. Blue indicators (y-axis) depict MSTO-211H and show that this cell line presented with changes in phosphorylation during cisplatin therapy. MSTO-211H cells have a distinct phosphorylation pattern compared to the other MPM cell lines. During therapy, a slight shift towards the phosphorylation pattern of the other two MPM cell lines can be seen. NCI-H2052 (shown in red) and NCI-H2452 (shown in yellow) present with an overlap in their phosphorylation pattern and show a much stronger phosphorylation of multiple phosphosites during therapy. Contrarily, MSTO-211H cells showed intermediate to slightly elevated phosphorylation. In contrast, MRC-5 cells present in general with minor phosphorylation regardless of the respective phosphosite and treatment.