Journal of Oncology

Research Article

Screening of Pleural Mesothelioma Cell Lines for Kinase Activity May Identify New Mechanisms of Therapy Resistance in Patients Receiving Platin-Based Chemotherapy

Table 1

Significant phosphorylation changes after cisplatin treatment ( values and Bonferroni-adjusted values).
Phosphosites valueBonferroni-adj. value
Tyrosine-protein phosphatase nonreceptor type 11 (EC 3.1.3.48) (protein-tyrosine phosphatase 2C) (PTP-2C) (PTP-1D) (SH-PTP3) (SH-PTP2) (SHP-2) (Shp2)._PTN11_57_67_Q061241.58E − 101.40E − 08
Gamma-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydrolyase) (neural enolase) (neuron-specific enolase) (NSE) (enolase 2)_ENOG_37_49_P091042.23E − 101.99E − 08
Tyrosine-protein phosphatase nonreceptor type 6 (EC:3.1.3.48)_PTN6_531_541_P293506.41E − 095.71E − 07
Paxillin._PAXI_24_36_P490238.74E − 097.78E − 07
Tyrosine-protein kinase JAK2 (EC 2.7.10.2) (janus kinase 2) (JAK-2)_JAK2_563_577_O606743.28E − 082.92E − 06
Embryonal fyn-associated substrate (HEFS)_EFS_246_258_O432813.38E − 083.01E − 06
Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell membrane protein 3_HAVR2_257_267_Q8TDQ04.21E − 083.75E − 06
Proto-oncogene tyrosine-protein kinase fes/Fps (EC 2.7.10.2) (C-Fes)_FES_706_718_P073325.51E − 084.91E − 06
B-cell antigen receptor complex-associated protein alpha-chain precursor (Ig-alpha) (MB-1 membrane glycoprotein) (surface IgM-associated protein) (membrane-bound immunoglobulin-associated protein) (CD79a antigen)_CD79 A_181_193_P119125.72E − 085.09E − 06
Tyrosine-protein kinase FRK (EC 2.7.10.2) (FYN-related kinase) (nuclear tyrosine protein kinase RAK)_FRK_380_392_P426856.73E − 085.99E − 06
NA_ART_004_EAIYAAPFAKKKXC_NA7.47E − 086.65E − 06
Tyrosine-protein kinase JAK1 (EC 2.7.10.2) (janus kinase 1) (JAK-1)_JAK1_ 1027_1039_P234588.05E − 087.17E − 06
Insulin receptor substrate 2_IRS2_626_638_Q9Y4H2 9.51E − 088.47E − 06
Hepatocyte growth factor receptor precursor (EC 2.7.10.1) (HGF receptor) (scatter factor receptor) (SF receptor) (HGF/SF receptor) (Met proto-oncogene tyrosine kinase) (c-Met)_MET_1228_1240_P085811.22E − 071.08E − 05
Paxillin._PAXI_111_123_P490231.42E − 071.26E − 05
Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase p85 subunit alpha) (PtdIns-3-kinase p85-alpha) (PI3K)_P85 A_600_612_P279861.81E − 071.61E − 05
Proto-oncogene tyrosine-protein kinase receptor ret precursor (EC 2.7.10.1) (C-ret)_RET_1022_1034_P079491.89E − 071.68E − 05
1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 (EC 3.1.4.11) (phosphoinositide phospholipase C) (PLC-gamma-1) (phospholipase C-gamma-1) (PLC-II) (PLC-148)_PLCG1_764_776_P191742.18E − 071.94E − 05
Protein tyrosine kinase 2 beta (EC 2.7.10.2) (focal adhesion kinase 2) (FADK 2) (proline-rich tyrosine kinase 2) (cell adhesion kinase beta) (CAK beta) (calcium-dependent tyrosine kinase) (CADTK) (related adhesion focal tyrosine kinase) (RAFTK)_FAK2_572_584_Q142892.55E − 072.27E − 05
Linker for activation of T-cells family member 1 (36 kDa phospho-tyrosine adapter protein) (pp36) (p36-38)_LAT_249_261_O435612.63E − 072.34E − 05
Mast/stem cell growth factor receptor kit (EC:2.7.10.1), CD117_KIT_930_942_C942S_P107212.91E − 072.59E − 05
Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-associated protein) (syk-related tyrosine kinase)_ZAP70_313_325_P434032.92E − 072.60E − 05
Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23), cell division protein kinase 1, cell division control protein 2 homolog, p34 protein kinase (CDK1)_CDK1_9_21_P064932.94E − 072.61E − 05
Erythropoietin receptor precursor (EPO-R)_EPOR_361_373_P192354.82E − 074.29E − 05
1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC:3.1.4.11) (phosphoinositide phospholipase C-gamma-2) (PLC-IV) (phospholipase C-gamma-2) (PLC-gamma-2)_PLCG2_1191_1203_C1200S_P168855.38E − 074.79E − 05
Platelet endothelial cell adhesion molecule precursor (PECAM-1) (EndoCAM) (GPIIA′) (CD31 antigen)_PECA1_708_718_P162845.64E − 075.02E − 05
Platelet endothelial cell adhesion molecule precursor (PECAM-1) (EndoCAM) (GPIIA′) (CD31 antigen)_PECA1_706_718_P162845.64E − 075.02E − 05
Cyclin-dependent kinase 2 (EC:2.7.11.22) cell division protein kinase 2 (EC 2.7.11.22) (p33 protein kinase)_CDK2_8_20_P249416.07E − 075.40E − 05
RAC-alpha serine/threonine-protein kinase (EC:2.7.11.1) (PKB, RAC)_AKT1_320_332_P317498.65E − 077.70E − 05
Ephrin type-A receptor 7 precursor (EC 2.7.10.1) (tyrosine-protein kinase receptor EHK-3) (EPH homology kinase 3) (receptor protein-tyrosine kinase HEK11)_EPHA7_607_619_Q153758.96E − 077.98E − 05
Vascular endothelial growth factor receptor 2 precursor (EC 2.7.10.1) (VEGFR-2) (kinase insert domain receptor) (protein-tyrosine kinase receptor Flk-1) (CD309 antigen)_VGFR2_989_1001_P359681.15E − 060.00010243
Myelin protein zero-like protein 1_MPZL1_236_246_O952971.16E − 060.00010287
Tyrosine-protein phosphatase nonreceptor type 6 (EC:3.1.3.48)_PTN6_558_570_P293501.30E − 060.00011555
Proto-oncogene tyrosine-protein kinase FER (EC 2.7.10.2) (p94-FER) (c-FER) (tyrosine kinase 3)_FER_707_719_P165911.47E − 060.00013057
Epidermal growth factor receptor precursor (EC 2.7.10.1) (receptor tyrosine-protein kinase ErbB-1)_EGFR_1165_1177_P005331.68E − 060.00014992
Paired mesoderm homeobox protein 2 (PRX-2) (paired-related homeobox protein 2)_PRRX2_202_214_Q998111.84E − 060.00016357
3-Phosphoinositide-dependent protein kinase 1 (EC 2.7.11.1) (hPDK1)_PDPK1_2_14_O155302.07E − 060.00018412
Receptor tyrosine-protein kinase erbB-2 precursor (EC 2.7.10.1) (p185erbB2) (C-erbB-2) (neu proto-oncogene) (tyrosine kinase-type cell surface receptor HER2) (MLN 19) (CD340 antigen)_ERBB2_870_882_P046262.73E − 060.00024328
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e_CD3E_182_194_P077662.84E − 060.00025288
3-Phosphoinositide-dependent protein kinase 1 (EC 2.7.11.1) (hPDK1)_PDPK1_369_381_O155303.02E − 060.00026873
Hepatocyte growth factor receptor precursor (EC 2.7.10.1) (HGF receptor) (scatter factor receptor) (SF receptor) (HGF/SF receptor) (Met proto-oncogene tyrosine kinase) (c-Met)_MET_1227_1239_P085813.42E − 060.0003046
Ras GTPase-activating protein 1 (GTPase-activating protein) (GAP) (Rasp21 protein activator) (p120GAP) (RasGAP)_RASA1_453_465_P209364.01E − 060.00035652
B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, CD272_BTLA_252_262_Q7Z6A94.20E − 060.00037375
40S ribosomal protein S6 (phosphoprotein NP33)_RS6_228_240P627531.57E − 050.00139465
Early E1A 32 kDa protein_E1A_ADE05_212_224P032552.12E − 050.00188822
Tyrosine-protein kinase JAK3 (EC:2.7.10.2) (janus kinase 3) (JAK-3)_JAK3_974_986_P523332.20E − 050.00196214
cAMP-dependent protein kinase type II-beta regulatory subunit_KAP3_107_119P313232.76E − 050.00245874
Insulin receptor substrate 1_IRS1_890_902_P355683.07E − 050.00272913
Ephrin type-A receptor 2 precursor (EC 2.7.10.1) (tyrosine-protein kinase receptor ECK) (epithelial cell kinase)_EPHA2_765_777_P293177.62E − 050.00677916
Proto-oncogene tyrosine-protein kinase LCK (EC 2.7.10.2) (p56-LCK) (lymphocyte cell-specific protein-tyrosine kinase) (LSK) (T-cell-specific protein-tyrosine kinase)_LCK_387_399_P062390.000122330.01088757
T-cell surface glycoprotein CD3 zeta chain precursor (T-cell receptor T3 zeta chain) (CD247 antigen)_CD3Z_77_89_P209630.000131280.01168433
Protein 4.1 (Band 4.1) (P4.1) (EPB4.1) (4.1 R)_41_654_666_P111710.00013310.01184597
Pleckstrin (platelet p47 protein)_PLEK_106_118P085670.000133590.01188922
Tyrosine-protein kinase ZAP-70 (EC 2.7.10.2) (70 kDa zeta-associated protein) (syk-related tyrosine kinase)_ZAP70_485_497_P434030.000141760.01261638
Ephrin type-A receptor 1 precursor (EC 2.7.10.1) (tyrosine-protein kinase receptor EPH)_EPHA1_774_786_P217090.000191040.01700258
Glycogen synthase kinase-3 beta (EC:2.7.11.26), serine/threonine-protein kinase GSK3B (EC:2.7.11.1)_GSK3B_210_222_C218S_P49841 0.000304040.0270594
Receptor tyrosine-protein kinase erbB-2 precursor (EC 2.7.10.1) (p185erbB2) (C-erbB-2) (neu proto-oncogene) (tyrosine kinase-type cell surface receptor HER2) (MLN 19) (CD340 antigen)_ERBB2_679_691P046260.000410110.0364998
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Raf-1) (C-RAF) (cRaf)_RAF1_332_344_P040490.000511470.04552087