Use of Antimetastatic SOD3-Mimetic Albumin as a Primer in Triple Negative Breast Cancer

Figure 3

PNA treated tumors have reduced tumor perfusion time and increased relative blood volume in the metastatic TNBC 4T1 model. Ultrasound imaging was used to visualize tumor microvasculature. The contrast agent was administered intravenously, images were acquired, and perfusion time was calculated with manufacturer software. PNA or HSA control was administered intravenously. And after 30 minutes, a second dose of contrast agent was administered, and perfusion time was calculated. (a) PNA treated tumors have reduced tumor perfusion time compared to HSA control. Tumor perfusion time was calculated by taking the 30-minute perfusion time minus the baseline perfusion time. (b) The kinetics of perfusion as measured by echo power versus time indicate PNA treated tumors have higher echo power at peak perfusion time compared to HSA control, indicating increased blood flow within the tumor. (c) Tumors were outlined in ultrasound B-mode (left panel). Parametric imaging of relative blood volume is shown 30 minutes after drug injection at the time of peak perfusion (right panel). HSA treated tumors show little to no signal intensity in the core of the tumor. (d) PNA treated tumors show greater relative blood volume within the tumor core.

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