N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines

Figure 1

In vitro and in situ PABA (N)-acetylation activity of parental and NAT1 KO clones for MDA-MB-231, MCF-7, and ZR-75-1 cell lines. (a) The in vitro PABA N-acetyltransferase activity in MDA-MB-231, MCF-7, and ZR-75-1 parental (P) and gRNA #2 (2) and #5 (5) clones NAT1 KO cell lines are shown. (b) The in situ PABA N-acetylation in MDA-MB-231, MCF-7, and ZR-75-1 parental (P) and gRNA #2 (2) and #5 (5) clones NAT1 KO cell lines are shown. Each bar illustrates mean ± SEM. Three or four separate determinations were performed in triplicate. ND is nondetectable ((a) <0.05 nmoles/min/mg; (b) <0.20 nmoles/hr/million cells).

(a)

(b)