N-Acetyltransferase 1 Knockout Elevates Acetyl Coenzyme A Levels and Reduces Anchorage-Independent Growth in Human Breast Cancer Cell Lines

Figure 2

NAT1 and NAT2 protein expression in breast cancer cell lines. (a). Relative NAT1 protein expression was evaluated following an in-cell western staining protocol as described in Materials and Methods. NAT1 protein expression was significantly () decreased in MDA-MB-231 gRNA #2 (2) and gRNA #5 (5) NAT1 KO cells compared to the parental (P); MCF-7 gRNA #2 (2) and gRNA #5 (5) and ZR-75-1 gRNA #2 (2) NAT1 KO cells compared to the respective parental (P) cells. (b). Relative NAT2 protein expression in MDA-MB-231 gRNA #2 (2) and gRNA #5 (5) KO cells was significantly () increased compared to the parental (P) cells, the same phenomenon was observed for MCF-7 gRNA #2 (2) and gRNA #5 (5) KO cells () compared to the parental (P); however, no significant () changes in the relative NAT2 protein expression were observed in ZR-75-1 gRNA #2 (2) NAT1 KO cells compared to the parental (P). Data expressed as mean ± SEM for 4-different determinations , , , .

(a)

(b)