TP53 Mutations Promote Immunogenic Activity in Breast Cancer

Figure 7

Mutant p53 promotes migration and proliferation of NK cells cocultured with MCF-7 cells. (a) NK92 cells cocultured with p53-mutant MCF-7 cells show stronger migration ability than NK92 cells cocultured with p53-wildtype MCF-7 cells, evident by transwell migration assay. (b) NK92 cells cocultured with p53-mutant MCF-7 cells show stronger proliferation ability than NK92 cells cocultured with p53-wildtype MCF-7 cells, evident by EdU proliferation assay. (c) Cytokines CCL4 and CCL5 have markedly higher levels in the serum containing NK92 cells cocultured with p53-mutant MCF-7 cells than in the serum containing NK92 cells cocultured with p53-wildtype MCF-7 cells, evident by quantitative enzyme-linked immunosorbent assay (ELISA).

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