Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells

Figure 2

Proteasomal inhibitor (ALLN) induces apoptosis. (a) Null-P53, wt-P53, and R273H-P53 cells were exposed to varying doses of ALLN. After 48 h of exposure, cell viability was measured through MTT assay. Untreated samples were taken as control. and # indicate significant difference compared to Null-P53 and R273H-P53 cells, respectively. (b) Percentage of apoptotic cells was measured through flow cytometry using Annexin V/PI staining and compared between wt-P53 and R273H-P53 cells upon ALLN treatment. (c) R273H-P53 cells were either untreated or treated with ALLN for 48 h and then stained with DAPI. Nuclear fragmentation or condensation after treatment is marked with white arrows. The scale bar represents 100 μm.

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