Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells

Figure 7

P53 regulates induction of autophagy in R273H-P53 cells. (a) Immunoblot analysis depicting P53 expression upon ALLN treatment with or without 30 min of prior PFT-α exposure. (b) MTT assay was performed after ALLN and/or PFT-α treatment for 48 h and percentage viability represented in the form of a bar diagram. (c) R273H-P53 cells were exposed to ALLN and/or PFT-α for 48 h and autophagy was checked through immunoblot analysis of Atg-5 (i) and P62 (ii). (d) MDC fluorescence assay was conducted after R273H-P53 cells were exposed to ALLN or PFT-α or in combination for 48 h. indicates significant difference compared to untreated control, while # indicates significant difference compared to ALLN-treated cells. “@” indicates significant difference compared to PFT-α-treated cells.

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