Autophagy Regulated by Gain of Function Mutant p53 Enhances Proteasomal Inhibitor-Mediated Cell Death through Induction of ROS and ERK in Lung Cancer Cells

Figure 8

R273H-P53 cells show ROS and ERK accumulation after PFT-α treatment. ((a) and (b)) R273H-P53 cells were exposed to ALLN and/or PFT-α (30 min prior to ALLN treatment) with and without NAC (1h prior to ALLN treatment) for 48 h and then cell viability and ROS levels were measured through (a) MTT assay and (b) DCFDA fluorimetric assay. ((c) and (d)) Cell viability and ROS levels were analyzed with/without 2 h prior treatment of U0126 and the result is shown in the form of a bar diagram. (e) Immunoblot analysis showing ph-ERK expression on ALLN and/or PFT-α treatment in R273H-P53 cells. indicates significant difference compared to untreated control and # indicates significant difference compared to ALLN-treated cells, while $$$ indicates the statistical difference compared to minus(-) NAC.

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