Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2

Figure 6

TRPV4 functionally mediated melanoma cells apoptosis via AKT pathway and was influenced by calcium. (a) Western blot analysis of MAPK as well as AKT signals was collected for activation of TRPV4 in A375 melanoma cells. Cells were treated by GSK1016790A with 20 nM and 50 nM for 24 h and lysates were then probed with the indicated antibodies, β-actin was used as a loading control, and pAKT was observed upregulated. (b) Assessments of pAKT changes triggered by GSK1016790A with 20 nM and 50 nM within 10 min as well as 24 h in melanoma A375 cells. (c) Influences of calcium ion on GSK1016790A-induced, TRPV4-mediated signal transduction. A375 cells were untreated or pretreated with EGTA or BAPTA-AM for 1 h before being stimulated with GSK1016790A. All tests were performed in at least three independent experiments.

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