Research Article

IL-2 Induces Transient Arrest in the G1 Phase to Protect Cervical Cancer Cells from Entering Apoptosis

Figure 3

Effect of IL-2 on cervical cancer cell apoptosis. HeLa (a) and INBL (b) cells were incubated in the presence or absence of IL-2 for 48 hours. Cells were fixed and incubated in the presence of annexin-V and evaluated by flow cytometry. No significant apoptosis was observed in either cell line. Puromycin-induced apoptosis was used as a positive control (c). Dot plots show apoptosis percentage after propidium iodide and FITC-Annexin V flow cytometry analysis on a FACSAria II cytometer (d). Representative flow cytometry plots of one out of three independent apoptosis assays.
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