Tubal Origin of “Ovarian” Low-Grade Serous Carcinoma: A Gene Expression Profile Study

Figure 3

Validation of differentially expressed genes by RT-PCR. Gene expression determined by microarray analysis and quantitative RT-PCR for genes up- and downregulated in the fallopian tube and ovarian samples (OVGP1, WT-1, FMO3, ARX, and FCN1). Compared to OSE samples, the gene expression level of OVGP1(A), WT-1(B), and FMO3(C) was significantly higher in samples of FTE, OEI, SC, SBT, and LGSC (p < 0.0001), while no statistical differences were detected among the samples of FT, OEI, SC, SBT, and LGSC. Compared to FT, OEI, SC, SBT, and LGSC samples, the expression level of ARX(D) and FCN1(E) was significantly higher in OSEs (p < 0.0001), while no statistical differences were detected among the samples of FT, OEI, SC, SBT, and LGSC. FTE, fallopian tube epithelia; OEI, ovarian epithelial inclusions; SC, serous cystadenoma; SBT, serous borderline tumor; LGSC, low-grade serous carcinoma; OSE, ovarian surface epithelia.