High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers

Figure 3

2D gel electrophoresis and western blot analysis with cell lysates from A549 and MCF-7 cell lines using the polyclonal anti-RTNKEASI serum and preimmune serum. Protein extract from A549 and MCF-7 cells were loaded in 2D gel followed by electrophoresis and transferred to PVDF for immunoblotting with the polyclonal anti-RTNKEASI serum or preserum. A rectangle marks the location of the ALDHs detected with the polyclonal anti-RTNKEASI serum in A549 cells; the reactive spots indicated with arrows were absent in MCF-7 cells and in the blot incubated with the preserum. These protein spots at 55 kDa, excited and subjected to MS analysis, correspond to ALDH1A1.