High Aldehyde Dehydrogenase Levels Are Detectable in the Serum of Patients with Lung Cancer and May Be Exploited as Screening Biomarkers

Table 2

Mass spectrometric identification of aldehyde dehydrogenase as 55 kDa target of the anti-RTNKEASI serum.

Gene symbol	Protein description	Unique peptides	Score	MW (kDa)
55 kDa band from A549 cell lysate excised from 1D gel
ALDH1A1	Retinal dehydrogenase	26	687	55.4
ALDH3A1	Aldehyde dehydrogenase, dimeric NADP-preferring	15	407	50.8
UGDH	UDP-glucose 6-dehydrogenase	3	140	55.7
GSR	Glutathione reductase mitochondrial	4	134	56.8
PDIA3	Protein disulfide-isomerase A3	3	93	57.1
ALDH2	Aldehyde dehydrogenase, mitochondrial	3	83	56.9
55 kDa band from A549 supernatant excised from 1D gel
ALDH1A1	Retinal dehydrogenase 1	29	742	55.5
G6PD	Glucose-6-phosphate 1-dehydrogenase	26	599	59.7
ALDH3A1	Aldehyde dehydrogenase, dimeric NADP-preferring	10	303	50.8
55 kDa band from A549 immunoprecipitation excised from 1D gel
ALDH1A1	Retinal dehydrogenase 1	28	351	55.5
G6PD	Glucose-6-phosphate 1-dehydrogenase	12	223	59.7
ALDH3A1	Aldehyde dehydrogenase, dimeric NADP-preferring	3	70	50.8
55 kDa spot 1 from A549 lysate excised from 2D gel
ALDH3A1	Aldehyde dehydrogenase, dimeric NADP-preferring	20	515	50.4
CCT2	T-complex protein 1 subunit beta	18	100.8	57.5
ALDH1A1	Retinal dehydrogenase 1	12	57.4	54.8
ALDH1B1	Aldehyde dehydrogenase X, mitochondrial	11	52	57.2
55 kDa spot 2 from A549 lysate excised from 2D gel
ALDH3A1	Aldehyde dehydrogenase, dimeric NADP-preferring	19	494.8	50.4
ALDH1A1	Retinal dehydrogenase 1	19	120.45	54.8

MW: molecular weight.