Danusertib induces evident changes in cell and nuclei morphologies in sensitive GSCs. (a) Representative images taken by phase contrast microscopy of GBM2, G179, and G144 cell lines treated with 500 nM Danusertib for 24, 48, and 72 h are reported. Drug treatment induced a significant increase in cell size starting from 48 h drug exposure. Scale bar = 100 μm. (b) Representative images of different nuclei shapes detected after 500 nM Danusertib treatment for 48 h and Giemsa staining. Sensitive cell lines highlighted the presence of a huge number of large multinucleated (A) and micronucleated (B) cells and polymorphic nuclei, such as ring-shaped nuclei (C). Scale bar = 100 μm. Quantitative data are reported in the graph. For statistical analysis, see Supplementary Table S2.