2-Methoxyestradiol and Its Combination with a Natural Compound, Ferulic Acid, Induces Melanoma Cell Death via Downregulation of Hsp60 and Hsp90

Figure 3

(a) Total cell levels in the early apoptosis phase after 24 hours incubation of A375 line cells with 10 μM 2-ME and 1 mM FA separately or in combination. (b) Total cell level in the late phase of apoptosis after 24 hours incubation of A375 line cells with 10 μM 2-ME and 1 mM FA separately or in combination. (c) Total cell level in the necrotic phase after 24 hours incubation of A375 with 10 μM 2-ME and 1 mM FA separately or in combination. Values are the mean ± SE from three independent experiments. No error bar means the thickness of the line is greater than the error. compared with the vehicle. The data were analyzed using GraphPad Prism Software version 6.02, performing one-way ANOVA analyses followed by Tukey’s multiple comparison test. , , , and vs. control.

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