GATA1 Promotes Gemcitabine Resistance in Pancreatic Cancer through Antiapoptotic Pathway
Figure 4
GATA1 regulates Bcl-XL transcription through binding to its promoter. (a) Luciferase reporter assay of CFPAC-1 and PANC-1 cells cotransfected with the Bcl-XL promoter-Luc reporter and control siRNA or GATA1 siRNAs. Representative Western blot analysis indicates the expression of GATA1. (b) Relative luciferase activity of different truncated Bcl-XL promoter reporter constructs in CFPAC-1 cells transfected with empty vector or GATA1. A, B, C, D, and E indicate putative binding sites of GATA1. (c) Relative luciferase activity of wild-type and mutated Bcl-XL promoter reporter constructs in CFPAC-1 cells transfected with empty vector or GATA1. B, C, and D indicate putative binding sites of GATA1. The “X” symbol denotes mutated GATA1-binding sites. (d) ChIP analysis of the occupancy of GATA1 protein on putative GATA1-binding sites. All values shown are means ± SD of three independent experiments with triplicate each, ; versus corresponding control.