MicroRNA-181a Inhibits Activated B-Cell-Like Diffuse Large B-Cell Lymphoma Progression by Repressing CARD11

Figure 4

Effects of miR-181a and CARD11 overexpression on ABC-DLBCL cells in vitro. (a) Relative miR-181a levels in OCI-LY10 and U2932 cells transfected with vector-only (white bar) or CARD11-overexpressing plasmids with wild-type (red) or mutated (blue) miR-181a binding site. (b) Western blot analysis of CARD11 levels in in OCI-LY10 and U2932 cells transfected with vector-only (white bar) or CARD11-overexpressing plasmids with wild-type (red) or mutated (blue) miR-181a binding site. (c) CCK-8 assay showing cell proliferation status of OCI-LY10 (left panel) and U2932 (right panel) cells transfected with vector-only (black line) or CARD11-overexpressing plasmids with wild-type (red line) or mutated (blue line) miR-181a binding site. (d) Cell cycle analysis showing percent G0/G1, S, and G2/M phase cells in OCI-LY10 (left panel) and U2932 (right panel) cells transfected with vector-only (black bar) or CARD11-overexpressing plasmids with wild-type (red bar) or mutated (blue line) miR-181a binding site as analyzed by flow cytometry. (e) Flow cytometry analysis of percent apoptotic cells (AnnexinV⁺PI⁺) in OCI-LY10 (left) and U2932 (right) cells transfected with vector-only (black bar) or CARD11-overexpressing plasmids with wild-type (red bar) or mutated (blue bar) miR-181a binding site. (f) Transwell matrigel assay showing invasiveness of OCI-LY10 (left) and U2932 (right) cells transfected with vector-only (black bar) or CARD11-overexpressing plasmids with wild-type (red bar) or mutated (blue bar) miR-181a binding site. Note: and versus control group; versus Lv-miR-181a group; results are expressed as mean ± SEM of three independent experiments.