PKHB1 Tumor Cell Lysate Induces Antitumor Immune System Stimulation and Tumor Regression in Syngeneic Mice with Tumoral T Lymphoblasts

Figure 3

IFN-γ and IL-2 secretion by unprimed or primed T cells cocultured with L5178Y-R cells. (a) L5178Y-R cells were cocultured with unprimed T-lymphocytes (previously cocultured with unstimulated DCs-Control) or primed T-lymphocytes (previously cocultured with pulsed DCs-PKHB1-TCL) in a 1:5 tumor to effector ratio, for 24 h, and the supernatants were collected and assayed for (a) IFN-γ release by ELISA and (b) IL-4 and IL-2 release by FACS. Graphs represent the means (±SD) of three experiments performed independently.

(a)

(b)