Research Article
Role of 17β-Estradiol on Cell Proliferation and Mitochondrial Fitness in Glioblastoma Cells
Figure 6
E2 increases HO-1 expression and induces Nrf2 nuclear translocation. E2 treatment increases Nrf2 nuclear translocation following 24 h treatment when compared with untreated cells (Figures 6(a) and 6(b)). Consistently, HO-1, one of the Nrf2-targeted genes, is upregulated following E2 treatment () (Figure 6(c)). Immunofluorescence staining of Nrf2 (green) was performed in U87-MG human glioblastoma cells at basal condition (Figure 6(a)) and after 24 hours of treatment with E2 (Figure 6(b)). DAPI was used to stain the cell nucleus, and the scale bar is set as 10 μm. Gene expression analysis of HO-1 was performed after 24 hours of treatment with E2 (5 nM) in glioblastoma cells. The calculated value of 2‐ΔΔCt in untreated controls is 1. Data are expressed as mean ± SD of at least four independent experiments (Figure 6(c)). ; .
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