Circ_0072088 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Cancer by Absorbing miR-377

Figure 4

circ_0072088 acts as a sponge for miRNA-377. (a) Venn diagram showed nine common downstream target genes of circ_0072088 predicted by two databases (CircInteractome and starBase). (b) Expression alterations in downstream target genes after the downregulation of circ_0072088 in TE-13 cells. (c) Expression alterations in downstream target genes after the overexpression of circ_0072088 in ECA109 cells. (d) Schematic diagram of the binding site of circ_0072088-miRNA-377-VEGF. (e) RIP assays confirmed the binding of circ_0072088 and Ago2 protein in ECA109 and TE-13 cells. (f) RIP assays confirmed the binding of miRNA-377 and Ago2 protein in ECA109 and TE-13 cells. (g) The mutation site of dual-luciferase reporter plasmid of circ_0072088. (h) Dual-luciferase reporter assay was performed for the assessment of luciferase activity after cotransfection of circ_0072088-wt/circ_0072088-mut and miRNA-377 mimics. , .

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