Circ_0072088 Promotes Proliferation, Migration, and Invasion of Esophageal Squamous Cell Cancer by Absorbing miR-377

Figure 5

circ_0072088 acts as a sponge for miRNA-377 to target VEGF to further promote the migration of ESCC cells. (a–d) qRT-PCR and Western blot were employed for examining the effects of downregulation and upregulation of miRNA-377 on the VEGF gene and protein expression. (e) The regulatory role of circ_0072088 downexpression on the VEGF protein level was examined by Western blot. (f) Western blot was adopted for determining the regulatory effect of circ_0072088 overexpression on the VEGF protein level and the regulatory role of miRNA-377 mimic on the upregulation of VEGF induced by circ_0072088 overexpression. (g) Transwell assay was conducted for examining the functions of miRNA-377 overexpression on the migration ability of ECA109 cells with circ_0072088 overexpression. , , and .

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