circDENND4C Promotes Proliferation and Metastasis of Lung Cancer by Upregulating BRD4 Signaling Pathway

Figure 4

circDENND4C acts as a sponge to adsorb miR-141-3p, and miR-141-3p inhibits the proliferation and invasion of NSCLC. (a) miR-141-3p is determined as the target of circDENND4C through StarBase website analysis. (b) RT-qPCR analysis of the expression of miR-141-3p in NSCLC tissues. (c) Pearson correlation analysis was used to study the correlation between circDENND4C and miR-141-3p. (d) The expression of miR-141-3p in sh-Ctrl and sh-circDENND4C groups. (e) The luciferase method was used to study the direct interaction between circDENND4C and miR-141-3p. (f) Detection of transfection efficiency of miR-141-3p mimics and miR-141-3p inhibitor. (g) Verifying the effect of miR-141-3p mimic or inhibitor on the proliferation of A549 and H1299 cells through functional experiments. (h) Functional experiments verify the effect of miR-141-3p mimic or miR-141-3p inhibitor on the migration and invasion of A549 and H1299. ^∗∗P < 0.01.