MicroRNA-2355-5p Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma via Suppressing NISCH Expression

Figure 3

miR-2355-5p repressed NISCH expression by binding to its 3′-UTR. (a) Effects of miR-2355-5p on the luciferase activity of the reporter gene inserted downstream of the wildtype and mutated NISCH 3′-UTR in CAL27 cells. The sequence of the miR-2355-5p binding site within the 3′-UTR of NISCH and its corresponding mutation were indicated. (b) RNA pulldown assay was performed in CAL27 cells transfected with wildtype and mutated NISCH, followed by qRT-PCR to detect the abundance of NISCH and miR-2355-5p. Data are presented as the mean ± standard deviation (SD). ns: not significant; .

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