Journal of Oncology / 2021 / Article / Fig 4

Research Article

MicroRNA-2355-5p Promotes the Proliferation of Head and Neck Squamous Cell Carcinoma via Suppressing NISCH Expression

Figure 4

Effect of miR-2355-5p on cell cycle progression in CAL27 and SCC25 cells. (a) Effects of miR-2355-5p mimics and inhibitor on miR-2355-5p expression in CAL27 and SCC25 cells. (b) The viability of each group of cells was detected by the CCK-8 assay. (c) For each group, cell cycle distribution was detected by flow cytometry analysis. (d) EdU staining (red) was performed to check the proliferating cells. Cell nuclei were stained with DAPI (blue). (e) Levels of NISCH, cyclin D1, and p-RbSer807 were detected by western blotting analysis in each group, with ß-actin as the reference protein. .

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