LncRNA PTPRG-AS1 Promotes the Metastasis of Hepatocellular Carcinoma by Enhancing YWHAG

Figure 2

Downregulation of PTPRG-AS1 attenuated proliferation and invasion of hepatocellular carcinoma in vitro. (a) qRT-PCR was employed to examine the quantification of PTPRG-AS1 in HepG2, PLC-PRF-5 transfected with LV-shPTPRG-AS1 and LV-NC. (b) CCK-8 assay was used to investigate cell viabilities of HepG2 and PLC-PRF-5 transfected with LV-shPTPRF-AS1 and LV-NC. (c) EdU analysis was employed to test the proliferation of HepG2 and PLC-PRF-5 transfected with LV-shPTPRG-AS1 and LV-NC. (d) Transwell assay was used to estimate the invaded cell numbers of HepG2 and PLC-PRF-5 transfected with LV-shPTPRG-AS1 and LV-NC. (e) Transwell assay was used to estimate the migrated cell numbers of HepG2 and PLC-PRF-5 transfected with LV-shPTPRG-AS1 and LV-NC. mRNA quantification of E-cadherin (f) and vimentin (g) in HepG2 transfected with LV-shPTPRG-AS1 and LV-NC were examined by qRT-PCR analysis. , , .

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