Journal of Oncology / 2021 / Article / Fig 4

Research Article

Yap-Hippo Signaling Activates Mitochondrial Protection and Sustains Breast Cancer Viability under Hypoxic Stress

Figure 4

Overexpression of HIF1 abolishes Yap knockdown-mediated breast cancer cell death and proliferation arrest. (a) MTT assay for cell viability in MCF-7 cells. siRNA against Yap (si-Yap) was transfected into cells before 24 hours hypoxia. HIF1 adenovirus (ad-HIF1) was transfected into Yap-deleted MCF-7 cells before 24 hours hypoxia. (b) TUNEL staining was used to observe the cell apoptotic rate. (c) CCK-8 assay was used to observe the cell proliferation capacity in MCF-7 cells under hypoxia. (d, e) qPCR assay was used to detect the transcription of VEGF and p21 in MCF-7 cells transfected with si-Yap. .

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