Journal of Oncology / 2021 / Article / Fig 2

Research Article

Heat Killed Salmonella typhimurium Protects Intestine Against Radiation Injury Through Wnt Signaling Pathway

Figure 2

HKST promoted proliferation in intestinal organoids and in vivo postirradiation. Intestinal crypts were isolated from mice small intestine and then seeded into Matrigel. Upon IR exposure, photos of intestinal organoids were taken after intestinal crypts were cultured after 7 days (a). Surface area and bud height of intestinal organoids were measured with image J software and analyzed with GraphPad prism 5, in which 30 organoids were calculated for each group (b, c). Values were given as mean ± SEM, represents the HKST + IR group versus IR group. The proliferation of intestinal organoids and crypts was detected by immunofluorescence staining of EDU (d) and Ki67 (f), respectively. The fluorescence intensity of EDU in organoids was measured by image J software and analyzed with GraphPad prism 5 (e). Values were given as mean ± SEM (n = 6), represents the HKST + IR group versus IR group.
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